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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
adeno- associated virus
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078mg/ml
- 规格:
96T
Human adeno- associated virus ELISA Kit
Product Name:Human adeno- associated virus ELISA Kit
Packing:96T
Catalog No.:ELA-E1314h
Gene Name:AAV
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:adeno-associated virus
Alternative Name:AAV,adeno-associated virus
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human adeno- associated virus ELISA Kit allows for the in vitro quantitative determination of adeno-associated virus concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human adeno- associated virus ELISA Kit has been pre-coated with an adeno-associated virus antibody specific to adeno-associated virus .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for adeno-associated virus and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain adeno-associated virus, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of adeno-associated virus in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Adeno-Associated Virus Biology
Adeno-associated virus (AAV) was first discovered as a contaminant of adenovirus stocks in the 1960s. The development of recombinant AAV vectors (rAAV) was facilitated by early studies that generated infectious molecular clones, determined
Generation of Recombinant Adeno-Associated Virus
Adeno-associated virus (AAV) was discovered about 30 yr ago as a contaminant of adenovirus preparations. Since its discovery, researchers have described many unique characteristics of AAV biology that have made it attractive
Targeted Integration by Adeno-Associated Virus
The integration of foreign DNA into the genomes of host cells is of fundamental importance for viral oncology, evolution, transgenic organisms, and gene therapy applications. Expression of foreign genes in eukaryotic cells is highly dependent
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