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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
WNT1 Inducible Signaling Pathway Protein 3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078mg/ml
- 规格:
96T
Human WNT1 Inducible Signaling Pathway Protein 3 ELISA Kit
Product Name:Human WNT1 Inducible Signaling Pathway Protein 3 ELISA Kit
Packing:96T
Catalog No.:ELA-E12936h
Gene Name:WISP3
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:WNT1 Inducible Signaling Pathway Protein 3
Alternative Name:WISP3,WNT1 Inducible Signaling Pathway Protein 3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human WNT1 Inducible Signaling Pathway Protein 3 ELISA Kit allows for the in vitro quantitative determination of WNT1 Inducible Signaling Pathway Protein 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human WNT1 Inducible Signaling Pathway Protein 3 ELISA Kit has been pre-coated with an WNT1 Inducible Signaling Pathway Protein 3 antibody specific to WNT1 Inducible Signaling Pathway Protein 3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for WNT1 Inducible Signaling Pathway Protein 3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain WNT1 Inducible Signaling Pathway Protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of WNT1 Inducible Signaling Pathway Protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验TRAF‐Mediated TNFR‐Family Signaling
map of the human TNF‐alpha/NF‐kappa B signal transduction pathway. Nat. Cell Biol. 6:97‐105. Brockhaus, M., Schoenfeld, H.J., Schlaeger, E.J., Hunziker, W., Lesslauer, W
Overview of Protein Microarrays
: Applications to signalling pathways and human body arrays. Brief. Funct. Genom. Proteom. 1:305‐315. Chen, C.‐S. and Zhu, H. 2006. Protein microarrays. BioTechniques 40:423‐429
interferon response elements in the promoter region, which are required for binding of the interferon-stimulated gene factor 3 (ISGF3) complex and for janus kinase/signal transducer of transcription (JAK/STAT)-pathway-mediated signaling. Expression of PRGs
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