- ￥2890
- LSM Bio
- 0.31-20ng/ml
- 0.1ng/ml
- 国产(香港）
- ELA-E1205h
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
SH2- containing protein
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human SH2- containing protein ELISA Kit
Product Name:Human SH2- containing protein ELISA Kit
Packing:96T
Catalog No.:ELA-E1205h
Gene Name:SHC(SLP-76
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:SH2-containing protein
Alternative Name:SHC(SLP-76,SH2-containing protein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SH2- containing protein ELISA Kit allows for the in vitro quantitative determination of SH2-containing protein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SH2- containing protein ELISA Kit has been pre-coated with an SH2-containing protein antibody specific to SH2-containing protein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SH2-containing protein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SH2-containing protein , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of SH2-containing protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of SH2 Ligands and Identification of Sites of Interaction
complexes. Src homology 2 (SH2) domains are protein modules of approx 100 amino acids that recognize and bind to specific phosphotyrosine-containing polypeptide sequences (1 ). SH2 domains are recruited to tyrosine-phosphorylated proteins in their normal
SH2 Domain-Based Tyrosine Phosphorylation Array
Tyrosine phosphorylation plays a major role in intracellular signal transduction pathways. Phosphorylated tyrosine residues initiate signaling pathways by recruiting proteins containing Src homology-2 (SH2) domains. Herein is described a high
BIAcore Analysis to Test Phosphopeptide-SH2 Domain Interactions
long and the three-dimensional structure of several SH2-containing molecules has been resolved. As it is precisely controlled by a subtle balance between protein tyrosine kinases (which phosphorylate) and protein tyrosine phosphatases
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