- ￥2890
- LSM Bio
- 0.31-20ng/ml
- 0.1ng/ml
- 国产
- ELA-E0930h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
phospho- extracellular signal- regulated kinase
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human phospho- extracellular signal- regulated kinase ELISA Kit
Product Name:Human phospho- extracellular signal- regulated kinase ELISA Kit
Packing:96T
Catalog No.:ELA-E0930h
Gene Name:pERK
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:phospho-extracellular signal-regulated kinase
Alternative Name:pERK,phospho-extracellular signal-regulated kinase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human phospho- extracellular signal- regulated kinase ELISA Kit allows for the in vitro quantitative determination of phospho-extracellular signal-regulated kinase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human phospho- extracellular signal- regulated kinase ELISA Kit has been pre-coated with an phospho-extracellular signal-regulated kinase antibody specific to phospho-extracellular signal-regulated kinase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for phospho-extracellular signal-regulated kinase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain phospho-extracellular signal-regulated kinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of phospho-extracellular signal-regulated kinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Activity Assays for Extracellular Signal-Regulated Kinase 5
Extracellular signal-regulated kinase 5 (ERK5) is also known as big MAPK (BMK1) or MAPK7. ERK5 is 115 kDa in mass and therefore larger than the other MAPKs such as ERK1/2, JNK, and p38. Like other MAPKs, ERK5 is ubiquitously expressed
The Extracellular Signal-Regulated Kinase (ERK) Cascade in Neuronal Cell Signaling
The Ras-controlled extracellular signal-regulated kinase (ERK) pathway mediates a large number of cellular events, from proliferation to survival, from synaptic plasticity to memory formation. In order to study the role
Recently, it has been shown that enzymes of the cyclic adenosine monophosphate (cAMP)-specific phosphodiesterase (PDE) family 4 can be directly phosphorylated by extracellular signal-regulated kinase 2 (ERK2). Phosphorylation of PDE4s by ERK
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