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- 详细信息
- 文献和实验
- 技术资料
- 灵敏度:
0.078ng/mL
- 库存:
50
- 供应商:
LSM Bio
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中Human Heparan sulfate, HS蛋白浓度
- 适应物种:
人
- 标记物:
Human Heparan sulfate, HS
- 样本:
血清、血浆或其它相关生物液体中天然和重组蛋白
- 规格:
96T
Human Heparan sulfate, HS ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Heparan Sulfate, HS
Search name
Human Heparan sulfate ELISA Kit, Human HS ELISA Kit, Heparan sulfate ELISA Kit, HS ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Heparan Sulfate concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan sulfate. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for Heparan sulfate and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Heparan sulfate, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heparan sulfate in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Article: Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed swiss albino mice independent of renal lipid signaling
Author:Asmaa M.RezkabIslam A.A.E.-H.IbrahimaMona F.MahmoudaAmr A.A.Mahmouda1
Received 6 September 2020, Revised 12 October 2020, Accepted 28 October 2020, Available online 4 November 2020.
Chemico-Biological Interactions
Available online 4 November 2020, 109307
doi: doi.org/10.1016/j.cbi.2020.109307
Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake
reports have shown that efficient polyamine uptake depends on the presence of polyanionic, cell surface-associated heparan sulfate proteoglycans (HSPGs). In this chapter, we provide protocols for studying HSPG-mediated uptake of polyamines in various cell
Interaction Between Respiratory Syncytial Virus and Glycosaminoglycans, Including Heparan Sulfate
Glycosaminoglycans (GAGs), including heparan sulfate (HS), are expressed on the surface of nearly all cells, linked to transmembrane proteins. These GAGs are sulfated to varying extents, lending a negative charge, and are used by a large
Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors
) are essential for animal development and homeostasis, and are involved in various pathological processes. The functions of HSPGs are largely exerted through interaction of the heparan sulfate (HS) side chains with different types of ligands, including diverse
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