- ￥2890
- LSM Bio
- 0.31-20ng/ml
- 0.1ng/ml
- 国产(香港）
- ELA-E0589h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
heme oxygenase 2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human Heme oxygenase 2, HO-2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Heme oxygenase 2,HMOX2,HO-2,HO2,heme oxygenase 2; heme oxygenase (decycling) 2
Search name
Human Heme oxygenase 2 ELISA KIT ,Human HMOX2 ELISA KIT ,Human HO-2 ELISA KIT ,Human HO2 ELISA KIT ,Human heme oxygenase 2 ELISA KIT ,Human heme oxygenase (decycling) 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human heme oxygenase 2,HO-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HO-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HO-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HO-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR
A variety of oxidative stress-inducing agents, such as metals, ultra-violet (UV) light, heme, and hemoglobin have been implicated in the pathogenesis of the inflammatory process. The cellular response to such agents involves the production
Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
demonstrated a specific upregulation of Hsp70 mRNA and protein after heat shock, while heme oxygenase 1 (HO1 or Hsp32) mRNA and protein was specifically upregulated following H2 O2 -induced oxidative stress. Hsp70 and HO1 should therefore be considered
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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