- ￥2890
- LSM Bio
- 0.31-20ng/ml
- 0.1ng/ml
- 国产
- ELA-E0587h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Cyclin- D3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human Cyclin- D3 ELISA Kit
Product Name:Human Cyclin- D3 ELISA Kit
Packing:96T
Catalog No.:ELA-E0587h
Gene Name:Cyclin-D3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Cyclin-D3
Alternative Name:Cyclin-D3,Cyclin-D3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cyclin- D3 ELISA Kit allows for the in vitro quantitative determination of Cyclin-D3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cyclin- D3 ELISA Kit has been pre-coated with an Cyclin-D3 antibody specific to Cyclin-D3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cyclin-D3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-D3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cyclin-D3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
to detect cyclin D1 expression in several tumor cell lines, activated human lymphocytes and chick chondrocytes. Below, HL-60 cells labeled for cyclin D1 or cyclin D2/D3 with 80% EtOH treatment at –20℃. References Gong, J., Bhatia, U
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
expression in several tumor cell lines, activated human lymphocytes and chick chondrocytes. Below , HL-60 cells labeled for cyclin D1 or cyclin D2/D3 with 80% EtOH treatment at �20°C. References Gong, J., Bhatia, U., Traganos, F
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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