Human Lysyl tRNA Synthetase, K

Human Lysyl tRNA Synthetase, KARS ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

KARS,CMTRIB; DFNB89; KARS1; KARS2; KRS; lysRS; lysine tRNA ligase; lysine--tRNA ligase; lysyl-tRNA synthetase

Search name

Human KARS ELISA KIT ,Human CMTRIB ELISA KIT ,Human DFNB89 ELISA KIT ,Human KARS1 ELISA KIT ,Human KARS2 ELISA KIT ,Human KRS ELISA KIT ,Human lysRS ELISA KIT ,Human lysine tRNA ligase ELISA KIT ,Human lysine--tRNA ligase ELISA KIT ,Human lysyl-tRNA synthetase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Lysyl tRNA Synthetase, KARS concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Lysyl tRNA Synthetase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lysyl tRNA Synthetase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Lysyl tRNA Synthetase in the samples is then determined by comparing the O.D. of the samples to the standard curve.