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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
zinc finger E-box binding homeobox 1
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
- 克隆性:
单克隆
- 适应物种:
Human,Mouse ,Rat
- 保质期:
12 month
- 库存:
50
- 供应商:
LSM bio
- 宿主:
Rabbit
- 应用范围:
ELISA,WB,IP
- 浓度:
≥95% as determined by SDS-PAGE
- 抗体英文名:
anti-ZEB1 antibody,ZEB1 antibody
- 抗体名:
anti-ZEB1 抗体,ZEB1 抗体
- 规格:
100μg
ZEB1抗体| ZEB1 antibody
货号 PAab09620
蛋白别名 AREB6 antibody, BZP antibody, DELTA EF1 antibody, delta EF1 antibody, antibody, Negative regulator of IL2 antibody, NIL 2 A antibody, NIL 2 A zinc finger protein antibody, NIL 2A antibody, NIL2A antibody, TCF 8 antibody, TCF8 antibody, Transcription factor 8 antibody, ZEB antibody, ZEB1 antibody, ZFHEP antibody, ZFHX1A
蛋白介绍
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
产品描述
anti-ZEB1 antibody is a Rabbit Polyclonal antibody againstZEB1..
建议稀释比例
WB : 1:500-1:5000;IP:1:200-1:2000
IHC
Western blot
MCF7 cells were subjected to SDS PAGE followed by western blot with PAab09620(ZEB1 antibody) at dilution of 1:500(本抗体仅供体外科研用途,不可用于临床诊断!)
武汉维克赛思科技有限公司
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文献和实验。(图片来源:Neuroblastoma is composed of two super-enhancer associated differentiation states) 转录因子ZEB1在基因上的结合分布(图片来源:Zeb1 potentiates genome-wide gene transcription with Lef1 to promote glioblastoma cell invasion)
表达,并与肝癌的侵袭特征成正相关,指示着肝癌病人更差的预后。存在于肝癌细胞中的lncRNA-ATB通过竞争性结合miR-200家族,上调其靶基因ZEB1和ZEB2,然后诱导EMT和肝癌细胞的体内外侵袭。虽然体内外实验都表明lncRNA-ATB的促侵袭作用依赖于其对miR-200家族的竞争性吸附,但是lncRNA-ATB促转移的作用并不能完全被miR-200所阻断。因此该课题组进一步利用RNA免疫共沉淀-深度测序(课题相关RIP-SEQ服务由上海伯豪生物技术有限公司提供)的方式,在全基因组范围内筛选
和hsa-miR-17*,但是在一篇最新(09.5.4)的gene development上一篇文章(The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2)中用到的是hsa-miR-17-3p,呵呵,这个到底有没有差异我想应该是没有的。应该最后会统一的吧,或者说他们真正的有差别,但是我不知道,呵呵。希望有知道
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