- ￥2260
- LSM Bio
- 进口
- PAab09270
- 2025年07月10日
- ELISA,WB,IHC,IF,IP
- E. coli - derived recombinant protein
- Human,Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
UPF1 regulator of nonsense transcripts homolog (yeast)
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,WB,IHC,IF,IP
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
UPF1 regulator of nonsense transcripts homolog (yeast)
- 抗体英文名：
anti-UPF1 antibody,UPF1 antibody
- 抗体名：
anti-UPF1 抗体,UPF1 抗体
- 规格：
100μl
UPF1抗体| UPF1 antibody
货号 PAab09270
蛋白别名 ATP dependent helicase RENT1 antibody, FLJ43809 antibody, FLJ46894 antibody, HUPF1 antibody, KIAA0221 antibody, NORF1 antibody, pNORF1 antibody, RENT1 antibody, UPF1
蛋白介绍
RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1- eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.
产品描述
anti-UPF1 antibody is a Rabbit Polyclonal antibody againstUPF1..
建议稀释比例
WB : 1:500-1:5000;IHC：1:20-1:200；IF:1：20-1：200;IP:1：200-1：2000
IHC
Immunohistochemistry of paraffin-embedded human kidney slide using PAab09270( UPF1 Antibody) at dilution of 1:50
Western blot
HepG2 cells were subjected to SDS PAGE followed by western blot with PAab09270(UPF1 antibody) at dilution of 1:1000(本抗体仅供体外科研用途，不可用于临床诊断!) "">
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文献和实验摘要 In both prokaryotes and eukaryotes nonsense mutations in a gene can enhance the decay rate of the mRNA transcribed from that gene, a phenomenon described as nonsense-mediated mRNA decay. In yeast, the products of the UPF1 and UPF3 genes
rna的调控过程方面取得了不少突破。在《BMC分子生物学》杂志上发表的一篇文章中，Jin和华中农业大学的同事们加深了我们对无意义介导的mRNA衰变机制的理解。无意义介导的mRNA衰变通过靶向含有提前终止密码子的mRNA来清除细胞内的非功能蛋白，否则这些mRNA将被翻译成截短的蛋白序列。这个系统中涉及的细胞机制还没有完全被理解，尽管已知转录调节蛋白UPF1通过识别异常翻译激活mRNA的衰变，通过使用包括SMG5蛋白在内的蛋白复合物。当SMG5被去磷酸化时，它下调了nonsense介导的mRNA衰退。Jin
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