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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
protein tyrosine phosphatase, non-receptor type 1
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse ,Rat
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
P67936
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB,IHC,IF,IP
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
protein tyrosine phosphatase, non-receptor type 1
- 抗体英文名:
anti-PTP1B antibody,PTP1B antibody
- 抗体名:
anti-PTP1B 抗体,PTP1B 抗体
- 规格:
100μg
PTP1B抗体| PTP1B antibody
货号 PAab06927
蛋白别名 PTP 1B antibody, PTP1B antibody, PTPN1
蛋白介绍
PTPN1(protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B, PTP-1B and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both insulin and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors(PMID:20101100). The gene might harbor variants influencing susceptibility to insulin resistance and type 2 diabetes(PMID:15919813).
产品描述
anti-PTP1B antibody is a Rabbit Polyclonal antibody againstPTP1B..
建议稀释比例
WB:1:500-1:5000;IP:1:200-1:2000;IHC:1:20-1:200;IF:1:10-1:100
IHC
Immunohistochemistry of paraffin-embedded human prostate cancer using PAab06927(PTPN1 antibody) at dilution of 1:50
Western blot
jurkat cells were subjected to SDS PAGE followed by western blot with PAab06927(PTPN1 antibody) at dilution of 1:300(本抗体仅供体外科研用途,不可用于临床诊断!) "">
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文献和实验Regulation of protein activity through the oxidation and reduction of cysteines is emerging as an important mechanism in the control of cell-signaling pathways. Protein tyrosine phosphatase 1B (PTP1B), for example, is reversibly inhibited
5% 脱脂奶粉,0.15 M NaClB:5% 脱脂奶粉封闭,一抗浓度:0.2 mg/mL,溶于 5% 脱脂奶粉,0.15 M NaClFig.1脱脂奶粉及 NaCl 浓度对实验结果的影响抗体:Human PTP1B Antibody (Catalog # AF1366)蛋白分离:人 HeLa 细胞 (Lane 1),MCF-7 (Lane 2) 和 TF-1 (Lane 3)(Fig.2)A. 5% 脱脂奶粉封闭,一抗浓度:1.0 mg/mL,溶于 2% 脱脂奶粉和 0.15 M NaClB
我是不是忘了告诉你,VEGF信号通路居然还有个从外到里套路……
2的内化,倒是挺别致的。VEGFR2在结合上一个VEGF后,会和另一个非酪氨酸激酶Neuropilin-1和synectin结合(对应的VEGFR2是酪氨酸激酶受体,RTK),然后包如膜体中,被内吞到细胞质里,然后VEGFR2自磷酸化后,激活更多下游信号通路:在转运过程中,Neuropilin-1在膜外会结合Rab5以及马达蛋白Myosin VI,然后沿着Actin转运:在转运过程中VEGFR2上的Y1175磷酸化位点附近会结合上PTP1b,遮蔽掉了ERK的激活位点,使之信号被抑制。但是转运
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