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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from human Histone H2A around the phosphorylation site of T121.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P0C0S8
- 抗体英文名:
Histone H2A (Phospho-Thr121) Polyclonal Conjugated Antibody
- 规格:
50ul/100ul
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
Chromatin Immunoprecipitation (ChIP)
. 16. Add relevant antibodies to the chromatin samples and rotate overnight at 4°C. Consult supplier’s information sheet for the amount of antibody to add. Typically, 5-10 µg is sufficient. IP Washes and Elution This portion
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