- 询价
- Signalway Antibody
- CHW213
- 2025年09月23日
- WB, IF
- Rabbit
- Hu,Ms,Rt
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Myosin.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Hu,Ms,Rt
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：P12882
- 抗体英文名：
Myosin-pan (acetyl-Lys505) Conjugated Antibody
- 规格：
50ul/100ul
计算分子量:223
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Intracellular Cytokine Staining Protocol
. Some protocols include a step of airfuging the antibody prior to multi-color flow analysis. For optimal performance of fluorochrome conjugated antibodies, wrap the vials with aluminum foil and store at 4°C in the dark. Do not freeze. When staining
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
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