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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Fusion protein corresponding to a region derived from internal residues of human Group-specific component (vitamin D binding protein)
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P02774
- 抗体英文名:
GC Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:53
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验是变性温度,预变性96度5分钟然后再加酶(热启动)一般循环的变性温度用95度比94好,还不行还可以再加高还有DMSO和甘油,各加到5%就足够了,光加DMSO有的时候会使酶的半衰期下降mcli:引物GC含量分别高达70%以上时,用普通PCR很难理想地扩增出此基因。因为G、C之间形成三对氢键,解链所需能量较高,故模板较难打开,在常规变性温度下,DNA模板变性不完全,同时由于单链的G+C丰富区易于自身互补配对形成稳定的发夹环二级结构,而使PCR引物难于结合到模板上,DNA聚合酶也难于延伸或停止延伸,结果常
0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated
the addition of deuterated internal standard THC-COOH-d9 into the sample followed by hydrolysis of conjugated THC-COOH by alkali. THC-COOH is extracted from urine or blood using liquid–liquid extraction followed by preparation of its trimethylsilyl derivatives
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