- ￥2062
- LSM Bio
- 进口
- PAab00952
- 2025年07月17日
- ELISA,IF,IHC
- E. coli - derived recombinant protein
- Human, Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
compound
- 亚型：
IgG2a
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Monoclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse ,Rat
- 保质期：
6个月
- 抗原来源：
Mouse
- 目录编号：
Q9GZT9
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,IF,IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
BrdU
- 抗体英文名：
anti-BrdU antibody,BrdU antibody
- 抗体名：
anti-BrdU 抗体,BrdU 抗体
- 规格：
100μg
BrdU抗体| BrdU antibody
货号 PAab00952
蛋白别名 5-bromo-2-deoxyuridine antibody, BrDu antibody, BrdUrd antibody, Bromodeoxyuridine antibody, BUdR
蛋白介绍
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
产品描述
anti-BrdU antibody is a Mouse Monoclonal antibody againstBrdU..
建议稀释比例
IHC:1:200-1:1000;IF:1:50-1:500
IHC
Immunohistochemistry of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using PAab00952( BrDu Antibody) at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Western blot
(本抗体仅供体外科研用途，不可用于临床诊断!)
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文献和实验a variety of cellular sources from human olfactory epithelium (Hahn et al., 2005) and neural progenitors (Nieoullon et al., 2005) to neural stem cells (Chu et al., 2004). BrdU antibody from Abcam Identification of the labelled cells can be applied
实验材料 Materials Required But Not Provided 1. BrdU reagent (Invitrogen Cat. No. 00-0103) 2. Cytospin Tubes 3. Phosphate-Buffered Saline 4. 0.22 micron filter 实验步骤 1. Labeling with BrdU In Vivo
Anti-BrdU-FITC (Becton Dickinson or Phoenix Flow) 0.15 M NaCl, 1.5 M NaCl 10 Paraformaldehype (kept as stock in -80°C) Tween 1M MgCl2 FACS Tubes Protocol: Cells in 96 well FACS plate Block with 24G-2 Surface stain cells
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