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文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain
Site-directed mutagenesis has become an extremely powerful means by which the nature and function of critical amino acids within a protein can be assessed and altered. However, the ability to exploit this technique requires that detailed
In recent years several techniques have been described for the introduction of DNA molecules into Escherichia coli . These are based on the findings of Mandel and Higa (1 ), who demonstrated that incubation of cells with naked bacteriophage DNA
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