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文献和实验Hemocytometer Nunc Freezing Container (# 5100-0001) Freezer Log Procedure The Master Cell Bank consists of 20 vials of cells at 5 x 106 cells/vial. Before preparing the Master Cell Bank, ensure that the cell line is stabilized
additional medium to suspension cultures to ensure that they are in log phase of growth. Chill the Freezing medium on ice. Label with cryotubes with the cell line name, the cells/vial, and the date.
Resuscitation of Frozen Cell Lines
clothing, remove ampule from container and place in a waterbath at an appropriate temperature for your cell line e.g. 37ºC for mammalian cells. Submerge only the lower half of the ampule. Allow to thaw until a small amount of ice remains in the vial
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