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文献和实验is added to the trypsinised cells. The cells are then centrifuged, resuspended in fresh culture medium and counted as above. This is especially necessary for serum-free cell culture. If a CO2 incubator is not available gas the flasks for 1-2
Quantitative Studies of Neuronal Chemotaxis in 3D
precisely controlled gradients of molecular factors within a three-dimensional culture environment. The method is based on a non contact mediated delivery of biomolecules to the surface of a collagen gel. The factors are printed in a pattern
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
Introduction In our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extracts. The cytoplasmic extracts may be prepared from cells which were treated in culture
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