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文献和实验TRICINE GELS Protocol (For Low MW proteins (
are very active in SDS sample buffer and can cause severe degradation. Once heated, sample could sit at RT for a short time until loading, or at -20°C for a long time. For a gel thickness of 0.75mm and 15 wells applied 10-25ug protein of a complex mixture
Biotinylation of Antibody ( Contributed by Nanci Donacki ) Antibody labeling (House Ear Institute) Labeling protocols using affinity markers. Coupling Colloidal Gold to Protein (Walter Steffen
• stabilizer soln (10% sodium thiosulfate+0.1N NaOH) • IODO-BEAD™(PIERCE) • Hamilton microsyringe (model 702, 25µl, needle gauge 22S, point style #2) • desalting column (Bio-rad DG-10 etc.) • column stand For procedure 2 • PBS
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