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- 详细信息
- 文献和实验
- 技术资料
- 库存:
141
- 英文名:
Recombinant Human Vascular Endothelial Growth Factor A
- 保质期:
3个月
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冻干蛋白可在-20℃长期
- 规格:
10μg|50μg|500μg|1mg
特别提示:包括重组人VEGF-A(VEGF165)在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人VEGF-A(VEGF165)
英文名称:Recombinant Human Vascular Endothelial Growth Factor A
产品货号:JN0224
产品规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物表达系统制备,目的基因编码的Ala27-Arg191表达纯化而来。
VEGF165质量控制:>95%(还原性SDS-PAGE)。
VEGF165制剂:冻干品
VEGF165保存:
冻干蛋白可在-20℃长期保存,室温也可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天。
复溶后建议分装冻存在-20℃,可保存3个月,应避免反复冻融。
VEGF165复溶:
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存,避免反复冻融。
关于VEGF165:
Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
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文献和实验permeability factor (VPF) in vivo, and a chemoattractant molecule for monocytes and endothelial cells. Five different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165
PriCells: Primary cells culture with supplement of vascular endothelial growth factor (VEGF)
8) splice site - the proximal splice site (denoted VEGFxxx) or distal splice site (VEGFxxxb). In addition, alternate splicing of exon 6 and 7 alters their heparin-binding affinity, and amino acid number (in humans: VEGF121, VEGF121b, VEGF145, VEGF165, VEGF165
干细胞扩增培养组分[2] 2.诱导多能干细胞(iPSC)向HSC分化 联合SCF、TPO、FGF basic、VEGF165等因子,可显著促进iPSC分化为造血干细胞样细胞(HSPC),高表达CD34、CD45等造血干细胞标志物,为再生医学、造血功能障碍治疗提供新的细胞来源。 图4.iPSC向红细胞的分化[3] 3.树突状细胞(DC)疫苗制备 DC细胞是肿瘤免疫治疗的核心抗原呈递细胞,在体外培养体系中添加GMP级重组人FLT3L,可高效扩增DC细胞前体,诱导DC细胞成熟
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