重组人MIF(带His标签)北京厂家

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产品名称：重组人MIF(带His标签)
英文名称：Recombinant Human Migration Inhibitor Factor, His
产品货号：GS4743
产品规格：10μg

分子量：Approximately 13.5kDa,a single non-glycosylated polypeptide chain containing 117 amino acids,with 6×His at the C-terminus.
储存条件：冷冻（-20℃）

概述

Migration Inhibitory Factor(MIF)is a secreted protein without a cleavable signal sequence and is secreted via a specialized,non-classical pathway.It is secreted by macrophages upon stimulation by bacterial lipopolysaccharide(LPS),or by M.tuberculosis antigens.MIF consists of two α-helices and six β-strands,four of which form a β-sheet.The two remaining β-strands interact with other MIF molecules,creating a trimer.Structure-function studies suggest MIF is bifunctional with segregated topology.The N- and C-termini mediate enzyme activity(in theory).Phenylpyruvate tautomerase activity(enol-to-keto)has been demonstrated and is dependent upon Pro at position 1.Amino acids 50-65(a.a.)have also been suggested to contain thiol-protein oxidoreductase activity.MIF has proinflammatory cytokine activity centered around 49 - 65(a.a.).On fibroblasts,MIF induces,IL-1,IL-8 and MMP expression;on macrophages,MIF stimulates NO production and TNF-α release folllowing IFN-γ activation.MIF apparently acts through CD74 and CD44,likely in some form of trimeric interaction.Human MIF is active on mouse cells.Human MIF is 90%,94%,95%,and 90%a.a.identical to mouse,bovine,porcine and rat MIF,respectively.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The specific activity is determined by binding rhCD74 in a functional ELISA.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素	Less than 1 EU/μg of rHuMIF,His as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFALEHHH HHH

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