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- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞类型：
其他细胞类型
- 细胞形态：
淋巴样
- 库存：
大量
- 年限：
50 years
- 运输方式：
冻存运输
- 生长状态：
悬浮生长，多细胞聚集
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2408™
| Designations: | NK-92MI | ||
| Depositors: | Conkwest Inc. | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | lymphoblast |
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| Source: | Disease: malignant non-Hodgkin's lymphoma Cell Type: natural killer cell; NK cell; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | D5S818: 12. 13 D13S317: 9, 12 D7S820: 10, 11 D16S539: 11, 12 vWA: 16, 18 THO1: 6, 9.3 TPOX: 8 CSF1PO: 11, 12 Amelogenin: X, Y |
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| Age: | 50 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. NK-92MI is an interleukin-2 (IL-2) independent Natural Killer Cell line derived from the NK-92 (ATCC CRL-2407 ) cell line by transfection. The parental cells were transfected with human IL-2 cDNA in the retroviral MFG-hIL-2 vector by particle-mediated gene transfer. The transfection is stable, most likely due to integration of the vector into genomic DNA. The cell line is cytotoxic to a wide range of malignant cells; it kills both K562 cells and Daudi cells in chromium release assays. NK-92 and derivative cell line NK-92MI have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR. The parental IL-2 dependent cell line is available as CRL-2407 (NK-92). NK-92MI was shown to contain, express, and synthesize the hIL-2. A culture submitted to the ATCC in September of 1998 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is Alpha Minimum Essential medium without ribonucleosides and deoxyribonucleosides but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate . To make the complete growth medium, add the following components to the base medium: 0.2 mM inositol; 0.1 mM 2-mercaptoethanol; 0.02 mM folic acid; horse serum to a final concentration of 12.5%; fetal bovine serum to a final concentration of 12.5%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Cultures can be maintained by centrifuging cells and resuspending cell pellet in fresh medium at 2 - 3 X 105 viable cells/mL. Centrifugation and full replacement of culture medium may be performed for the first subcultures. Cultures can then be maintained by addition of fresh medium. Pipet the cells up and down on the back of the flask every 2-3 days to produce a single cell suspension. Maintain cell density between 2 X 10 5 and 1 X 10 6 viable cells/ml or use a 1:3 spilt ratio. | ||
| Preservation: | Freeze medium: FBS, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum: ATCC 30-2020 recommended serum: ATCC 30-2040 parental cell line: ATCC CRL-2407 (EBV po |
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| References: | 38894: Gong JH, et al. Characterization of a human cell line (NK-92) with phenotypical and functional characteristics of activated natural killer cells. Leukemia 8: 652-658, 1994. PubMed: 8152260 38969: Tam YK, et al. Characterization of genetically altered, interleukin 2-independent natural killer cell lines suitable for adoptive cellular immunotherapy. Hum. Gene Ther. 10: 1359-1373, 1999. PubMed: 10365666 40184: Tam YK, et al. Immunotherapy of malignant melanoma in a SCID mouse model using the highly cytotoxic natural killer cell line NK-92. J. Hematother. 8: 281-290, 1999. PubMed: 10417052 |
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在NK-92细胞培养中，血清的选择和处理方式会影响培养基的配制。查看配方时，你可能会发现一个细节：马血清标注需要热灭活，而胎牛血清通常按配方要求直接加入。同样是血清，为什么操作不同？关键在于两者的来源、补体活性，以及配方对细胞培养条件的要求。下面将依次说明这一区别、马血清热灭活的原因、胎牛血清的处理方式，以及容易混淆的操作要点。实际配制时，应同时核对细胞株培养说明、培养基配方和所用血清的产品说明。
NK-92为人恶性非霍奇金淋巴瘤患者的自然杀伤细胞，属于IL-2依赖型NK细胞系。是NK细胞功能研究、CAR-NK工程化改造及免疫治疗机制探索的常用悬浮模式细胞，细胞聚团生长，边缘清晰、折光性强。细胞增殖依赖重组IL-2，缺少IL-2的细胞会在72 h内死亡。本文将针对NK-92的细胞培养流程进行梳理和指导。 一、细胞复苏 1.【准备工作】水浴锅提前预热至37℃；9mL完全培养基37℃预热；生物安全柜紫外照射灭菌30min； 2.【融化】液氮取出冻存管，37℃水浴快速摇晃融化，约1-2
, none of which can cause disease solely by themselves. To reveal the genetic bases of MI, we performed a large-scale, case-control association study using 92,788 gene-based single-nucleotide polymorphism (SNP) markers. We have identified functional SNPs
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NK-92MI
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