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- 详细信息
- 技术资料
- 器官来源：
肺
- 运输方式：
冻存运输
- 生长状态：
混合型生长
- 相关疾病：
其他疾病
- ATCC Number：
CRL-5845™
- 年限：
stage L
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | NCI-H841 [H841] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed: adherent and suspension, Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells. | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: lung Tumor Stage: stage L Disease: carcinoma; variant small cell lung cancer Derived from metastatic site: lymph node |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11 D13S317: 12 D16S539: 13 D5S818: 11,12 D7S820: 10,13 THO1: 6,9 TPOX: 11,12 vWA: 16,17 |
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| Cytogenetic Analysis: | t(2p,3p), t(3q,9q) | ||
| Age: | 51 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian, White | ||
| Comments: | The line was established in October 1984. The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 70. |
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| Propagation: | ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum
Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Add fresh medium as cell density increases. Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 |
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| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. | ||
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NCI-H841
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