- 询价
- 2026年05月29日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
其他疾病
- ATCC Number：
HTB-177™
- 生长状态：
贴壁生长
| Designations: | NCI-H460 [H460] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: lung Disease: carcinoma; large cell lung cancer Derived from metastatic site: pleural effusion |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1982 | ||
| Applications: | The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. |
||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 13 D16S539: 9 D5S818: 9,10 D7S820: 9,12 THO1: 9.3 TPOX: 8 vWA: 17 |
||
| Cytogenetic Analysis: | modal numbr = 57; range = 53 to 65. This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells. Three other markers were found in some cells only. The markers, t(7;9) and t(7;16) were mostly paired. Normal N9 was absent, and N7 and N16 had only a single copy per cell. Two copies each of the X and the Y were present in all cells. |
||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
||
| Gender: | male | ||
| Comments: | The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Twice per week |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Doubling Time: | 23 hrs in medium with serum; 42 to 60 hrs in serum | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
||
| References: | 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
询价记录
暂无询价记录
文献和实验相关实验
NCI-H526来源于一名55岁白人男性小细胞肺癌（Stage E）患者的骨转移灶，从肺部分离获得。该细胞高表达两个小细胞肺癌生化标志物——神经元特异性烯醇化酶和脑肌酸激酶同工酶，是肺癌研究中常用的体外模型之一。 形态特征与生长方式 悬浮生长，细胞以大量聚团形式存在。刚复苏时细胞是分散的，24-48小时后状态逐渐恢复，开始聚团生长。传代时不需要完全吹散成单细胞，把大团吹成小团即可，避免团块中间的细胞缺乏营养。 培养要点与注意事项 培养基选用1640添加10%胎牛血清及1%双抗
都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
NCI-H446人小细胞肺癌细胞培养全攻略：从基本信息到实操要点
做小细胞肺癌研究的朋友，对NCI-H446这个细胞系一定不陌生。这篇文章把它的来源、特性、培养要点都梳理了一遍，新手也能快速上手。 细胞系名称与来源 细胞名称：NCI-H446（人小细胞肺癌细胞），也常简称H446。 别称：H-446、NCI-446、NCIH446。 来源：1982年由Carney和Gazdar等人从一位61岁白人男性小细胞肺癌患者的胸腔积液中分离建系。值得一提的是，原始肿瘤的形态并不符合典型小细胞肺癌的特征。 细胞特性与应用场景 NCI-H446属于
技术资料暂无技术资料 索取技术资料
NCI-H460
询价
