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- 技术资料
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
其他疾病
- ATCC Number：
HTB-177™
- 生长状态：
贴壁生长
| Designations: | NCI-H460 [H460] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: carcinoma; large cell lung cancer Derived from metastatic site: pleural effusion |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1982 | ||
| Applications: | The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. |
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| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 13 D16S539: 9 D5S818: 9,10 D7S820: 9,12 THO1: 9.3 TPOX: 8 vWA: 17 |
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| Cytogenetic Analysis: | modal numbr = 57; range = 53 to 65. This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells. Three other markers were found in some cells only. The markers, t(7;9) and t(7;16) were mostly paired. Normal N9 was absent, and N7 and N16 had only a single copy per cell. Two copies each of the X and the Y were present in all cells. |
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| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
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| Gender: | male | ||
| Comments: | The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 23 hrs in medium with serum; 42 to 60 hrs in serum | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 |
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都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
人胃癌细胞(未分化) SNL-105 KATO III 人胃癌细胞 SNL-106 NCI-N87 人胃癌细胞 SNL-107 SNU-1 人胃癌细胞 SNL-173 MKN45 人低分化胃癌细胞 SNL-176 MKN74 人胃癌细胞 SNL-304 GES-1 人胃粘膜细胞 SNL-325 Hs-746T 人胃癌细胞 SNL-365 MFC 小鼠胃癌细胞 SNL
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NCI-H460
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