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- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 组织来源：
cortex
- 相关疾病：
其他疾病
- ATCC Number：
CRL-10296™
- 器官来源：
肾上腺
- 细胞形态：
上皮样
- 库存：
大量
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 年限：
48 years
| Designations: | NCI-H295 [H295] | ||
| Depositors: | AF Gazdar | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, with some loosely adherent cells, suspension with some loosely attached cells | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: adrenal gland Tissue: cortex Disease: adrenocortical carcinoma |
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| Cellular Products: | steroid hormones | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 13 D16S539: 11 D5S818: 12 D7S820: 9,12 THO1: 9.3 TPOX: 8 vWA: 17,18 |
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| Cytogenetic Analysis: | modal number = 62; range = 55 to 64; hypertriploid. Twenty-three marker chromosomes were common to most cells: der(2)t(2;18)(p25;p11), del(3)(q21), der(10)t(10;13)(p15;q11), der(7)t(7;7)(q36;p13), del(7)(q11),; der(8)t(7;8)(p13;p23), del(8)(p12), del(9)(p22), del(9)(p13), del(11)(p12), del(12)(p12), t(13q18q) and 11 others. The del(9)(p13) was paired; normal N13 was absent. N15 had three copies and N22 had four copies.; A total of 65 marker chromosomes were detected in 38 metaphases examined.; A minor subline that occurred in about 16% of the cells was characterized by the presence of del(M5)(7p11) replacing M5, by the single N17 (instead of paired N17) and by the presence of an additional M42. | ||
| Age: | 48 years | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| Propagation: | ATCC complete growth medium: HITES medium supplemented with 2% fetal bovine serum
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cells with some loosely attached cells. Shake the flask to dislodge the cells and transfer to a new flask. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with an additional 8% fetal bovine serum and 5% DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 |
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| References: | 23082: Gazdar AF, et al. Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res. 50: 5488-5496, 1990. PubMed: 2386954 | ||
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