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- 2026年01月02日
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- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 年限：
15 years
- 器官来源：
肝
- 生长状态：
贴壁生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
肝癌
- ATCC Number：
CRL-11997™
- 库存：
大量
| Designations: | HEP G2/2.2.1 | ||
| Depositors: | Northeastern Ohio University College of Medicine | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Disease: hepatocellular carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 9,13 D16S539: 12,13 D5S818: 11,12 D7S820: 10 THO1: 9 TPOX: 8,9 vWA: 17 |
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| Age: | 15 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Cell line was derived from the hepatocellular carcinoma cell line, HepG2 (ATCC HB-8065 ). The parental cells were stably transfected at passage 48 with a human cholesterol 7 alpha-hydroxylase (CYP7) minigene/Luciferase construct. | ||
| Propagation: | ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate and 1200 mg/L sodium bicarbonate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: These cells are slow to attach after subculture. Allow 4 to 5 days for reattachment. |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 parental cell line:ATCC HB-8065 |
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| References: | 40153: Chiang JY, Stroup D. Assay for agents that affect cholesterol 7alpha-hydroxylase expression and a characterization of its regulatory elements. US Patent 5,821,057 dated Oct 13 1998 | ||
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