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- 2026年01月02日
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- 年限：
8 years juvenile
- 器官来源：
肝
- 相关疾病：
肝癌
- ATCC Number：
HB-8064™
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B] | ||
| Depositors: | Wistar Institute | ||
| Biosafety Level: | 2 [Cells Contain HEPATITIS B ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Disease: hepatocellular carcinoma |
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| Cellular Products: | alpha fetoprotein (alpha-fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; [3525 ] alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C3, C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); [3525 ] alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein); Gc globulin [3525 ] |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 8 D13S317: 12,14 D16S539: 10 D5S818: 13 D7S820: 8,10 THO1: 6,7 TPOX: 9 vWA: 17 |
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| Cytogenetic Analysis: | modal number = 60 with a subtetraploid mode of 82; has a rearranged chromosome 1 [3525 ] | ||
| Age: | 8 years juvenile | ||
| Gender: | male | ||
| Ethnicity: | Black | ||
| Comments: | This line contains an integrated hepatitis B virus genome. [1205 ] [3525 ] | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 1205: Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960 3525: Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983 22909: Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137 24388: Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851 |
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文献和实验Approximately 5% of the world’s population has been infected with hepatitis B virus (HBV). Ten percent of these adults will become chronic carriers, as will 95% of the infants infected perinatally. Those that do become chronically infected
HepG2是p53野生型的。 如果你研究肝癌的话，那么告诉你----HuH7是p53突变型的、Hep3B是P53缺失的。 edwardellen doctormy wrote: HepG2是p53野生型的。 如果你研究肝癌的话，那么告诉你----HuH7是p53突变型的、Hep3B是P53缺失的。 很感谢！ 还想继续问一下，能从哪里查到这些信息
tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation 发表期刊：Immunity 影响因子：43.474（2022 年最新数据） 2.实验基础信息 样本信息： 对照组：WT 小鼠 实验组：除 WT（untreated）外，其它组小鼠（雄性）均出生后两周开始注射 DEN，后期每周注射 CCl4 以诱导肝癌的发生。 单细胞捕获平台：10X Genomics 单细胞实验分组：Miz1∆hep：Miz
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