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- 详细信息
- 询价记录
- 技术资料
- 相关疾病：
肿瘤
- ATCC Number：
HTB-118™
- 年限：
64 years
- 器官来源：
其他
- 库存：
大量
- 生长状态：
贴壁生长
- 细胞形态：
混合型
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 运输方式：
冻存运输
| Designations: | SW962 [SW 962, SW-962] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | mixed |
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| Source: | Organ: vulva Disease: carcinoma Derived from metastatic site: lymph node |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | Blood type A; Rh+ | ||
| Cytogenetic Analysis: | hypertriploid; modal number = 80; range = 70 to 85. The rate of higher ploidies was 4.3%., At least 15 marker chromosomes were common to most cells. These include: t(1p3p), t(1p17p), i(3q), t(8q9q), del(3)(p21), 20q+ and nine others. Of these, i(3q), t(8q9q), del(3)(p21) and a few others were generally paired., An additional segment on 20q+ is long, but its origin is unknown. The total length of 20q+ is as long as that of N1. Normal N3 was absent, and N1 and N18 had only a single copy per cell. Usually, there were three copies of normal X chromosomes. | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1 |
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| Age: | 64 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian. A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474 |
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SW 962
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