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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
腺癌
- ATCC Number：
CCL-235™
- 年限：
grade IV
- 细胞形态：
上皮样
- 库存：
大量
- 生长状态：
贴壁生长
- 器官来源：
直肠
- 运输方式：
冻存运输
| Designations: | SW837 [SW-837] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: rectum Tumor Stage: grade IV Disease: adenocarcinoma |
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| Cellular Products: | carcinoembryonic antigen (CEA) 523 ng/10 exp6 cells/10 days; keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | HLA A23, A32, B15, B35; blood type O; Rh+ | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 9,12 THO1: 9.3 TPOX: 8,9 vWA: 15,16 |
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| Isoenzymes: | ES-D, 1 G6PD, B PGD, A PGM1, 1 PGM3, 1 |
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| Age: | 53 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. There is a C -> T mutation in codon 248 of the p53 gene resulting in an Arg -> Trp substitution. The cells are positive for keratin by immunoperoxidase staining. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 1 to 2 times per week Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks. |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| References: | 22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 22260: Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989 22930: Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228 |
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SW837
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