- 询价
- 2025年12月31日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
腺癌
- ATCC Number：
CCL-235™
- 年限：
grade IV
- 细胞形态：
上皮样
- 库存：
大量
- 生长状态：
贴壁生长
- 器官来源：
直肠
- 运输方式：
冻存运输
| Designations: | SW837 [SW-837] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: rectum Tumor Stage: grade IV Disease: adenocarcinoma |
||
| Cellular Products: | carcinoembryonic antigen (CEA) 523 ng/10 exp6 cells/10 days; keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | HLA A23, A32, B15, B35; blood type O; Rh+ | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 9,12 THO1: 9.3 TPOX: 8,9 vWA: 15,16 |
||
| Isoenzymes: | ES-D, 1 G6PD, B PGD, A PGM1, 1 PGM3, 1 |
||
| Age: | 53 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. There is a C -> T mutation in codon 248 of the p53 gene resulting in an Arg -> Trp substitution. The cells are positive for keratin by immunoperoxidase staining. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0°C |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 1 to 2 times per week Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks. |
||
| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| References: | 22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 22260: Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989 22930: Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Homo sapiens (human) LoVo CCL-230 Homo sapiens (human) SW403 [SW-403] CCL-231 Homo sapiens (human) SW48 [SW-48] CCL-233 Homo sapiens (human) SW1116 [SW 1116, SW-1116] CCL-235 Homo sapiens (human) SW837 [SW-837] CCL-237 Homo sapiens
藤红素和 19-048 治疗后能抑制结直肠癌细胞的增殖以及降低细胞活力。为了确定雷公藤红素和 19-048 是否通过靶向 PRDX1 影响细胞氧化还原状态，作者在 SW620 和 HCT116 细胞中用 DCFH-DA 荧光探针染色检测 ROS 水平，在不同浓度的雷公藤红素和 19-048 处理后，与对照相比，细胞中的 ROS 含量急剧升高，随后检测了雷公藤红素和 19-048 对 NCM460 细胞中 ROS 水平的影响，结果表明，雷公藤红素和 19-048 对正常细胞 ROS 诱导的影响小于癌
「外泌体 + 环状 RNA」国自然双热点助力肿瘤研究发表高分文章
的外泌体能促进 CRC 的增殖、迁移和侵袭 作者从两种 CRC 细胞系（HCT116和SW480）上清中分别分离获得外泌体，经过透射电镜、NTA 和 WB 鉴定后，确认已分离出外泌体。随后，作者用外泌体处理对应的 CRC 细胞，发现外泌体能显著促进 CRC 细胞增殖、迁移和侵袭，并减少凋亡。 WB 结果也显示，外泌体能提升 CRC 细胞中 BCL-2、 N-cadherin、Vimentin、MMP9 蛋白水平，和降低 E-cadherin、Cleaved-caspase3、Cleaved
技术资料暂无技术资料 索取技术资料
