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- 文献和实验
- 技术资料
- 生长状态：
漂浮生长
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
肺
- 相关疾病：
小细胞肺癌
- ATCC Number：
CRL-5898™
- 年限：
stage L
- 运输方式：
冻存运输
| Designations: | NCI-H1836 [H1836] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | floating aggregates of round cell clusters | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: lung Tumor Stage: stage L Disease: carcinoma; classic small cell lung cancer |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 10,12 D16S539: 11,13 D5S818: 11 D7S820: 11,12 THO1: 8,9.3 TPOX: 9,10 vWA: 16 |
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| Cytogenetic Analysis: | del(p11-qter) | ||
| Age: | 52 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was established in February 1988. The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 38 pack years. |
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| Propagation: | ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum
Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium as cell density increases. Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation with subsequent resuspension in fresh medium. |
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| Preservation: | culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 |
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| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. | ||
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文献和实验一、实验材料NCI-H1299，购自上海晶莱生物技术有限公司。表 2.1.1 主要试剂试剂与耗材厂家（货号）细胞培养瓶FALCON 中国（353014）Penicillin/streptomycin solution（KGY002）0.25% Tripsin-EDTA（BK-E3076）PBS（BK330-2）胎牛血清GIBCO 美国（10270-106）1640 培养基Hyclone 美国（SH3080901）Annexin V-FITC/PI 凋亡试剂盒 二、实验仪器表 2.2.1 主要
2017 年 8 月 31 日，山东烟台毓璜顶医院呼吸内科邹慎春为第一作者和通讯作者 Yao Zhang 在《分子医学报告》（Molecular Medicine Reports）发表题为《Epicatechin induces apoptotic and autophagic cell death in NCI-H2172 non‑ small cell lung cancer cells along with concomitant mitochondrial membrane
Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs
into the lung cancer cell line NCI-H1299. The CPT–BB conjugate was metabolized by NCI-H1299 intracellular enzymes releasing the cytotoxic CPT. Receptor binding methods and fluorescent methods are presented for studying the internalization
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