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- 详细信息
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 组织来源：
duct
- 年限：
TNM stage IIIA, grade 2
- 库存：
大量
- 器官来源：
乳房
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 相关疾病：
导管癌
- ATCC Number：
CRL-2340™
- 生长状态：
悬浮生长
| Designations: | HCC2157 | ||
| Depositors: | AF Gazdar, AK Virmani | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates, Cells in suspension show an inclination to form duct-like structures and gland-like structures. | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: mammary gland; breast Tissue: duct Tumor Stage: TNM stage IIIA, grade 2 Disease: primary ductal carcinoma |
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| Cellular Products: | Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935. | ||
| Applications: | HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). The cells are positive for expression of Her2-neu and for expression of p53. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). |
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| Receptors: | estrogen receptor, negative progesterone receptor, positive |
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| Oncogene: | her2/neu +, p53 + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 11 D5S818: 13 D7S820: 10,12 THO1: 8,9 TPOX: 9,11 vWA: 14,18 |
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| Cytogenetic Analysis: | Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% | ||
| Age: | 48 years adult | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| Comments: | This cell line was initiated on 3/4/96 and took 8 months to establish. The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). The cells are positive for expression of Her2-neu and for expression of p53. HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341 . |
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| Propagation: | ATCC complete growth medium: ACL-4 medium supplemented with 10% FBS The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium. Cultures grow as floating aggregates of large irregular cell clusters. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 Medium additive:ATCC 30-2214 derived from same individual:ATCC CRL-2341 |
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| References: | 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 | ||
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HCC2157
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