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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 相关疾病：
非小细胞肺癌
- ATCC Number：
CRL-5893™
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞类型：
其他细胞类型
- 年限：
stage 4
- 器官来源：
肺
- 生长状态：
悬浮生长，多细胞聚集
| Designations: | NCI-H1770 [H1770] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: lung Tumor Stage: stage 4 Disease: carcinoma; non-small cell lung cancer Derived from metastatic site: lymph node Cell Type: neuroendocrine; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,13 D13S317: 13 D16S539: 12,13 D5S818: 11,12 D7S820: 11 THO1: 9 TPOX: 11 vWA: 18,19 |
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| Age: | 57 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian, White | ||
| Comments: | The line was established in November 1987. The tissue donor was a non-smoker. A lymphoblastoid line from the same patient is available as ATCC CRL-5960 . |
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| Propagation: | ATCC complete growth medium: ACL-4 medium (serum-free) The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium as cell density increases. Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cells grow as floating aggregates of round cell clusters. |
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| Preservation: | Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 normal (or near-normal) cell line established from the same patient:ATCC CRL-5960 |
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| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. | ||
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NCI-H1770
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