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- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 器官来源：
结肠
- 细胞形态：
上皮样
- 年限：
Dukes' type C, grade III
- 库存：
大量
- 相关疾病：
大肠癌
- ATCC Number：
CCL-230™
- 运输方式：
冻存运输
| Designations: | SW403 [SW-403] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: colon Tumor Stage: Dukes' type C, grade III Disease: colorectal adenocarcinoma |
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| Cellular Products: | carcinoembryonic antigen (CEA) 155 ng/10 exp6 cells/10 days; keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | blood type O | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,13 D13S317: 13 D16S539: 10,12 D5S818: 11 D7S820: 8,9 THO1: 6 TPOX: 8,9 vWA: 14,18 |
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| Cytogenetic Analysis: | modal number = 68; range = 59 to 73. The stemline chromosome number is near triploid with 2S component occurring at about 1.4% and 10 marker chromosomes were common to S metaphases. Except for monosomic 1, 13 and 22, and pentasomic 20, most normal chromosome types ranged from disomy to tetrasomy. The cell line is karyotypically very homogeneous and stable. | ||
| Isoenzymes: | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
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| Age: | 51 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | CSAp negative (CSAp-). Colon antigen 3, positive. The cells are positive for keratin by immunoperoxidase staining. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Two to three times weekly |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 |
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