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- 组织来源：
synovium
- 细胞形态：
混合型
- 库存：
大量
- 生长状态：
贴壁生长
- 年限：
25 years
- 相关疾病：
其他疾病
- ATCC Number：
HTB-93™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 运输方式：
冻存运输
| Designations: | SW 982 [SW-982, SW982] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | mixed |
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| Source: | Tissue: synovium Disease: synovial sarcoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 12,13 D16S539: 11,12 D5S818: 11,13 D7S820: 9,11 THO1: 9.3 TPOX: 9,11 vWA: 19,20 |
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| Cytogenetic Analysis: | Hyperdiploid; modal number = 48; range = 42 to 58. The rate of higher ploidies was 1.6%. Nine markers were common to all cells. These were: t(1q4p), del(5) (q31;q33), der(9) t(4;9) (q11;p24), t(8q12p), t(9q13q) and four others.Double minutes (DM) were seen in some cells (usually only one copy). Normal N9 was absent, N4, N8, and N13 were consistently single-copied and the X was double-copied. | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1-2 |
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| Age: | 25 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The SW 982 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a biphasic synovial sarcoma removed from a 25 year old female Caucasian. The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma. An ampule at passage 4 was received at the ATCC in January, 1982. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 100% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 | ||
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的外泌体能促进 CRC 的增殖、迁移和侵袭 作者从两种 CRC 细胞系（HCT116和SW480）上清中分别分离获得外泌体，经过透射电镜、NTA 和 WB 鉴定后，确认已分离出外泌体。随后，作者用外泌体处理对应的 CRC 细胞，发现外泌体能显著促进 CRC 细胞增殖、迁移和侵袭，并减少凋亡。 WB 结果也显示，外泌体能提升 CRC 细胞中 BCL-2、 N-cadherin、Vimentin、MMP9 蛋白水平，和降低 E-cadherin、Cleaved-caspase3、Cleaved
藤红素和 19-048 治疗后能抑制结直肠癌细胞的增殖以及降低细胞活力。为了确定雷公藤红素和 19-048 是否通过靶向 PRDX1 影响细胞氧化还原状态，作者在 SW620 和 HCT116 细胞中用 DCFH-DA 荧光探针染色检测 ROS 水平，在不同浓度的雷公藤红素和 19-048 处理后，与对照相比，细胞中的 ROS 含量急剧升高，随后检测了雷公藤红素和 19-048 对 NCM460 细胞中 ROS 水平的影响，结果表明，雷公藤红素和 19-048 对正常细胞 ROS 诱导的影响小于癌
腺癌，未见肝内占位效应（肝转移）。总之，AFPGC 是一类具有高度恶性病理学特征的胃癌，在临床工作中如果发现 AFP 升高，无肝脏及生殖腺病灶者，要考虑到胃癌的可能，临床上应加强对该类疾病的认识，做好筛查，应用多种手段，早发现，早治疗。致谢：感谢胃镜室唐利颖和病理科郝秀荣对本文相关图片的贡献。参考文献[1] 卢锦。原发性胃癌甲胎蛋白异常 1 例报道。检验医学与临床，2013,10 (13):1764-1765.[2]Chen WQ,Zheng RS,Zhang SW,et al.Report
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