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- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- 年限：
44 years
- 库存：
大量
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2170™
- 运输方式：
冻存运输
| Designations: | SW 1573 [SW-1573, SW1573] | ||
| Depositors: | W McCombs | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: alveolar cell carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432 | ||
| Antigen Expression: | Blood Type O; Rh + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 12,14 D5S818: 12,13 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 20 |
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| Age: | 44 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 100% |
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| Subculturing: | Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474 | ||
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文献和实验藤红素和 19-048 治疗后能抑制结直肠癌细胞的增殖以及降低细胞活力。为了确定雷公藤红素和 19-048 是否通过靶向 PRDX1 影响细胞氧化还原状态，作者在 SW620 和 HCT116 细胞中用 DCFH-DA 荧光探针染色检测 ROS 水平，在不同浓度的雷公藤红素和 19-048 处理后，与对照相比，细胞中的 ROS 含量急剧升高，随后检测了雷公藤红素和 19-048 对 NCM460 细胞中 ROS 水平的影响，结果表明，雷公藤红素和 19-048 对正常细胞 ROS 诱导的影响小于癌
「外泌体 + 环状 RNA」国自然双热点助力肿瘤研究发表高分文章
的外泌体能促进 CRC 的增殖、迁移和侵袭 作者从两种 CRC 细胞系（HCT116和SW480）上清中分别分离获得外泌体，经过透射电镜、NTA 和 WB 鉴定后，确认已分离出外泌体。随后，作者用外泌体处理对应的 CRC 细胞，发现外泌体能显著促进 CRC 细胞增殖、迁移和侵袭，并减少凋亡。 WB 结果也显示，外泌体能提升 CRC 细胞中 BCL-2、 N-cadherin、Vimentin、MMP9 蛋白水平，和降低 E-cadherin、Cleaved-caspase3、Cleaved
【求助】做细胞周期，G0G1期减少，S期增多，G2/M期变化不大说明什么
相同的，尤其是癌细胞。 jn1573 这个是药物效果的表现，具体原因还要从蛋白表达变化上去分析。和其它文献和报道的不一样是好事，这样才是发现新问题的好文章，只要自己能从不同的水平和角度分析原因就可以了。 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你，都可以成为师兄的好伙伴 师兄微信号：shixiongcoming
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