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- 2026年01月05日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞类型：
B淋巴细胞
- 运输方式：
冻存运输
- 年限：
3 years
- 相关疾病：
淋巴瘤
- ATCC Number：
CRL-1432™
- 生长状态：
悬浮生长
- 免疫类型：
IgM; lambda light chain
- 细胞形态：
淋巴样
- 库存：
大量
| Designations: | NAMALWA | ||
| Depositors: | NB Finter | ||
| Isotype: | IgM; lambda light chain | ||
| Biosafety Level: | 2 [Cells Contain Herpesvirus ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Disease: Burkitt's lymphoma Cell Type: B lymphocyte; |
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| Cellular Products: | immunoglobulin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 9 D5S818: 12,13 D7S820: 11 THO1: 7,9.3 TPOX: 6,11 vWA: 14 |
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| Cytogenetic Analysis: | This is a human cell line with the hypodiploid chromosome count. The modal chromosome number was 44, occurring in 30% of cells. The rate of cells with higher ploidies was 2.8%. Twelve to 14 marker chromosomes were common to most cells. Among these were ins(1;?) (q11;?), ins(3) (q11q29), del(3) (p12/13), der(6)t(3;6) (p21;p25), t(15q21q) and seven to nine others. Normal N3 was absent, and N7 had three copies per cell. The X chromosome was single. There was no Y chromosome. | ||
| Age: | 3 years | ||
| Gender: | female | ||
| Comments: | The cells contain the Epstein-Barr virus (EBV) genome. | ||
| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 92.5%; fetal bovine serum, 7.5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Cultures should be started at 5 X 10(5) viable cells/ml and subcultured at 2 X 10(6) cells/ml. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 1059: Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 22364: Klein G, et al. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44-57, 1972. PubMed: 4122458 58035: Nyormoi O, et al. Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Int. J. Cancer 12: 396-408, 1973. PubMed: 4365093 |
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- 作者
- 内容
- 询问日期
文献和实验抗体对细胞的毒性剂量 Toxic dose of antibiotics for cell lines
Namalwa 500 1500 Xeno AE3 200 S49 1000 S49 kin- 500
T细胞淋巴瘤 HCT-8 回盲肠腺癌 Namalwa Burkitt`s淋巴瘤 HCe-8693 盲肠未分化腺癌 J urknt.Clone E6-1 白血病细胞 HR-8348 直肠腺癌 THP-1 单核细胞 BEL-7402 肝癌 U937 组织细胞淋巴瘤 BEL- 7404 肝癌 Raji Burkitt's淋巴瘤 BEL-7405 肝癌 MEG-01 成巨核细胞白血病 HepG2 肝细胞癌
蛋白溶酶原激活剂、凝血因子Ⅷ和Ⅸ、促红细胞素、松弛素、生长激素、蛋白C、免疫球蛋白、尿激酶、激肽释放酶及200种单克隆抗体等。其中，口蹄疫疫苗是动物细胞大规模培养方法生产的主要产品之一。1983年，英国Wellcome公司就已能够利用动物细胞进行大规模培养生产口蹄疫疫苗。美国Genentech公司应用SV40为载体，将乙型肝炎病毒表面抗原基因插入哺乳动物细胞内进行高效表达，已生产出乙型肝炎疫苗。英国Wellcome公司采用8000L Namalwa细胞生产α干扰素。英国Celltech公司用气升式 生物
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