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- 运输方式：
冻存运输
- 相关疾病：
恶性黑色素瘤
- ATCC Number：
HTB-72™
- 年限：
51 years adult
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
皮肤
- 库存：
大量
- 细胞形态：
多边形
- 生长状态：
贴壁生长
| Designations: | SK-MEL-28 | ||
| Depositors: | T Takahashi | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | polygonal |
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| Source: | Organ: skin Disease: malignant melanoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | Blood Type A; Rh+; HLA A11, A26, B40, DRw4 | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 9,12 D5S818: 11,13 D7S820: 10,9.3 THO1: 7 TPOX: 8,12 vWA: 16,19 |
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| Cytogenetic Analysis: | modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell. | ||
| Isoenzymes: | AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1 |
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| Age: | 51 years adult | ||
| Gender: | male | ||
| Comments: | This is one of a very extensive series of melanoma lines (see also ATCC HTB-70 ,ATCC HTB-71 and ATCC HTB-73 ) isolated by T. Takahashi and associates. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 purified DNA:ATCC HTB-72D |
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| References: | 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 23256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619 |
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文献和实验相关实验
Sucrose Density Gradient Fractionation
below).Resuspend in 10 ml SK with 1 mg zymolyase and 28.8 mM Beta-mercaptoethanol to make SK:thaw zymolyase (kept at 5 mg/ml stock in SK at -20℃)at room temp,then spin 1 min at 14,000 rpm.Take supe.-- for 4 gradients,use 900μl of supe,90μl of beta-mercaptoethanol
Sucrose Density Gradient Fractionation of Yeast Membranes
., 10 min., 30oC in lab clinical centrifuge Wash 1x with 10 ml SK buffer in 15 ml falcon tubes (see recipe below). Resuspend in 10 ml SK with 1 mg zymolyase and 28.8 mM Beta-mercaptoethanol to make SK: thaw zymolyase (kept at 5 mg/ml
below). Resuspend in 10 ml SK with 1 mg zymolyase and 28.8 mM Beta-mercaptoethanol to make SK: thaw zymolyase (kept at 5 mg/ml stock in SK at -20oC) at room temp, then spin 1 min at 14,000 rpm. Take supe.-- for 4 gradients, use 900 ul of supe, 90 ul
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SK-MEL-28
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