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TOV-112D

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  • 2026年01月12日
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    • 询价记录
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    • 技术资料
    • 器官来源

      卵巢

    • 物种来源

    • 是否是肿瘤细胞

      1

    • 相关疾病

      其他疾病

    • ATCC Number

      CRL-11731™

    • 年限

      grade 3, stage IIIC

    • 库存

      大量

    • 运输方式

      冻存运输

    • 细胞形态

      上皮样

    • 生长状态

      贴壁生长

    Designations: TOV-112D
    Depositors:  University of Montreal
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens deposited as human
    Morphology: epithelial

    Source: Organ: ovary
    Tumor Stage: grade 3, stage IIIC
    Disease: primary malignant adenocarcinoma; endometrioid carcinoma
    Cellular Products: keratin [49408 ]
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Tumorigenic: Yes
    Oncogene: her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175)
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 12
    D13S317: 8
    D16S539: 9,12
    D5S818: 10
    D7S820: 9,10
    THO1: 6
    TPOX: 8,11
    vWA: 18
    Cytogenetic Analysis: 52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 [49408 ]
    Age: 42 years adult
    Gender: female
    Comments: This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer. [49408 ]
    Unlike TOV-21G (ATCC CRL-11730 ) and OV-90 (ATCC CRL-11732 ), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24. [42090 ]
    Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Temperature: 37.0°C
    Subculturing: Protocol: Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
    Medium Renewal: Every 3 to 4 days
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor temperature
    Related Products: recommended serum:ATCC 30-2020
    purified DNA:ATCC CRL-11731
    References: 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
    49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

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    图标文献和实验
    相关实验
    • Proteomic Profiling of Ovarian Cancer Models Using TMT-LC-MS/MS

      Herein, we have utilized two cellular models of epithelial ovarian cancer (EOC), where transfer of normal chromosome 18 material into the EOC cell lines TOV-112D and TOV-21G induced in vitro and in vivo suppression of tumorigenic phenotype

    •   ESCO-2185 ESCO 特殊扎带EA475HB-2 EA475HB-2

        ESCO-20130001 ESCO 插座EA940CH-2 EA940CH-2 CP-1310090072 ESCO 胶锤EA575D-25 EA575D-25 CP-1310090071 ESCO 胶锤EA575VH-60 EA575VH-60 ESCO-1310 ESCC 加长改锥EA550AK EA550AK ESCO-10041 ESCO 丝维套装EA829B EA829B ESCO-10040 ESCO 板手EA613

    •   ESCO-1520 ESCO 温度探头EA701CA-4 EA701CA-4

      109 EA109 ESCO-1520 ESCO 温度探头EA701CA-4 EA701CA-4 ESCO-1519 ESCO 割管刀(大)EA203C EA203C ESCO-1518 ESCO 力矩扳手EA723A-26 EA723A-26 ESCO-1517 ESCO 真空泵转接器EA112X-220 EA112X-220 ESCO-1516 ESCO 真空泵转接器EA112X-10 EA112X-10 ESCO-1515 ESCO 扩口器EA

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