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- 询价记录
- 文献和实验
- 技术资料
- 器官来源:
卵巢
- 物种来源:
人
- 是否是肿瘤细胞:
1
- 相关疾病:
其他疾病
- ATCC Number:
CRL-11731™
- 年限:
grade 3, stage IIIC
- 库存:
大量
- 运输方式:
冻存运输
- 细胞形态:
上皮样
- 生长状态:
贴壁生长
| Designations: | TOV-112D | ||
| Depositors: | University of Montreal | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: ovary Tumor Stage: grade 3, stage IIIC Disease: primary malignant adenocarcinoma; endometrioid carcinoma |
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| Cellular Products: | keratin [49408 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Oncogene: | her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175) | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 8 D16S539: 9,12 D5S818: 10 D7S820: 9,10 THO1: 6 TPOX: 8,11 vWA: 18 |
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| Cytogenetic Analysis: | 52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 [49408 ] | ||
| Age: | 42 years adult | ||
| Gender: | female | ||
| Comments: | This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer. [49408 ] Unlike TOV-21G (ATCC CRL-11730 ) and OV-90 (ATCC CRL-11732 ), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24. [42090 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 3 to 4 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | recommended serum:ATCC 30-2020 purified DNA:ATCC CRL-11731 |
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| References: | 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998 49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 |
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文献和实验Proteomic Profiling of Ovarian Cancer Models Using TMT-LC-MS/MS
Herein, we have utilized two cellular models of epithelial ovarian cancer (EOC), where transfer of normal chromosome 18 material into the EOC cell lines TOV-112D and TOV-21G induced in vitro and in vivo suppression of tumorigenic phenotype
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