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- 详细信息
- 技术资料
- 库存：
大量
- 年限：
9 months
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1440™
- 生长状态：
贴壁生长
- 器官来源：
肾脏
- 运输方式：
冻存运输
| Designations: | G-402 | ||
| Depositors: | PT Peebles | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial-like |
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| Source: | Organ: kidney Disease: renal leiomyoblastoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Cytogenetic Analysis: | This is a human cell line with the hyperdiploid chromosome count. The modal chromosome number was 47, occurring in 40% of cells. The rate of higher ploidies was 0.3%. The karyotype was 47,XX,+2,-5,+?ins(4) (q11.2) for the modal cell population. No other karyotypic abnormalities were evident. | ||
| Isoenzymes: | G6PD, B | ||
| Age: | 9 months | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line will grow in soft agar. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007 recommended serum:ATCC 30-2020 |
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G-402
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