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- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 相关疾病：
导管癌
- ATCC Number：
HTB-133™
- 组织来源：
duct
- 细胞形态：
上皮样
- 库存：
大量
- 器官来源：
乳房
- 年限：
54 years adult
- 运输方式：
冻存运输
| Designations: | T-47D | ||
| Depositors: | I Keydar | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: mammary gland; breast Tissue: duct Disease: ductal carcinoma Derived from metastatic site: pleural effusion |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Receptors: | calcitonin, expressed androgen receptor, expressed estrogen receptor, expressed progesterone receptor, expressed glucocorticoid receptor, positive, expressed prolactin, expressed calcitonin; androgen receptor, positive; progesterone receptor, positive; glucocorticoid; prolactin; estrogen receptor, positive |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,13 D13S317: 12 D16S539: 10 D5S818: 12 D7S820: 11 THO1: 6 TPOX: 11 vWA: 14 |
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| Cytogenetic Analysis: | This is a hypotriploid human cell line. The modal chromosome number is 65 occurring at 50% and polyploidy at 0.8%. 18 marker chromosomes are common to most cells, of which 7 are paired and 11 are single-copied. The t(8q14q), t(9q17q), t(10q17p) are among 7 paired markers common to most cells. N7, N9, and N10 are absent and N11 is generally present in 4 copies. DM's occurred, but infrequently. Q-band examination did not show the presence of a Y chromosome. | ||
| Isoenzymes: | AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1 |
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| Age: | 54 years adult | ||
| Gender: | female | ||
| Comments: | The cells express the WNT7B oncogene [PubMed: 8168088]. The T-47 line was isolated by I. Keydar from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast. This differentiated epithelial substrain (T-47D) was found to contain cytoplasmic junctions and receptors to 17 beta estradiol, other steroids and calcitonin. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.2 Units/ml bovine insulin; fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 32 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 purified DNA:ATCC 45528 purified DNA:ATCC 45529 purified DNA:ATCC HTB-133D purified RNA:ATCC HTB-133R |
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| References: | 1120: Judge SM, Chatterton RT Jr.. Progesterone-specific stimulation of triglyceride biosynthesis in a breast cancer cell line (T-47D). Cancer Res. 43: 4407-4412, 1983. PubMed: 6871874 1122: Lamp SJ, et al. Calcitonin induction of a persistent activated state of adenylate cyclase in human breast cancer cells (T-47D). J. Biol. Chem. 256: 12269-12274, 1981. PubMed: 6271778 1123: Sher E, et al. Whole-cell uptake and nuclear localization of 1,25-dihydroxy-cholecalciferol by breast cancer cells (T-47D) in culture. Biochem. J. 200: 315-320, 1981. PubMed: 6896147 1124: Freake HC, et al. 1,25-Dihydroxyvitamin D3 specifically binds to a human breast cancer cell line (T-47D) and stimulates growth. Biochem. Biophys. Res. Commun. 101: 1131-1138, 1981. PubMed: 6272774 23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 32244: Hoppe HC, et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed: 9284169 32915: Burfeind P, et al. Antisense RNA to the type I insulin-like growth factor receptor suppresses tumor growth and prevents invasion by rat prostate cancer cells in vivo. Proc. Natl. Acad. Sci. USA 93: 7263-7268, 1996. PubMed: 8692980 58052: Keydar I, et al. Establishment and characterization of a cell line of human breast carcinoma origin. Eur. J. Cancer 15: 659-670, 1979. PubMed: 228940 |
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文献和实验相关实验
Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
of in vitro models to understand how both biophysical and biochemical signals regulate complex cellular behaviors. T47D breast epithelial cells will differentiate into duct-like tubules when cultured in a floating three-dimensional (3D) collagen gel
L.T. Thompson, Ph.D. Aging Memory Research Labs
as needed. Pellets can also be weighed―if in doubt, weigh and record them daily as well. L.T. Thompson, Ph.D. Aging & Memory Research Labs, UT Dallas Rodent Behavior Protocols 2 • Clearly mark the cage "caloric restricted" or "food deprived
人的红细胞的寿命一般为120天。红细胞死亡后变成间接胆红素（I-Bil），经肝脏转化为直接胆红素（D-Bil），组成胆汁，排入胆道，医学教育网搜|索整理最后经大便排出。间接胆红素与直接胆红素之和就是总胆红素（T-Bil）。上述的任何一个环节出现障碍，均可使人发生黄疸。如果红细胞破坏过多，产生的间接胆红素过多，肝脏不能完全把它转化为直接胆红素，可以发生溶血性黄疸；当肝细胞发生病变时，或者因胆红素不能正常地转化成胆汁，或者因肝细胞肿胀，使肝内的胆管受压，排泄胆汁受阻，使血中的胆红素
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T-47D
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