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- 详细信息
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 库存：
大量
- 细胞形态：
上皮样
- 相关疾病：
肿瘤
- ATCC Number：
CRL-7920™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 器官来源：
宫颈
| Designations: | DoTc2 4510 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: cervix Disease: carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Ameloginin: X CSF1PO: 12 D13S317: 11 D16S539: 12, 13 D5S818: 14 D7S820: 11, 13 TH01: 7 TPOX: 8 vWA: 16, 17 |
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| Cytogenetic Analysis: | modal number = 62; range = 57 to 75 | ||
| Isoenzymes: | G6PD, B | ||
| Gender: | female | ||
| Comments: | Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. Please see the NBL Repository description. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 Trypsin EDTA Solution: ATCC 30-2101 |
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文献和实验KCl + 14.4 grams Na2HPO4 + 2.4 grams KH2PO4. pH upon dilution to 1X should be 7.2. BSA Sigma #A7638. Glutathione-Agarose Sigma# G-4510. Prepare according to instructions. Anti-GST Molecular Probes, Inc. #A5800. Rabbit IgG (H+L
+ 2 grams KCl + 14.4 grams Na2HPO4 + 2.4 grams KH2PO4. pH upon dilution to 1X should be 7.2. BSA Sigma #A7638. Glutathione-Agarose Sigma# G-4510. Prepare according to instructions. Anti-GST Molecular Probes, Inc. #A5800. Rabbit IgG (H+L
后适当处理。对MAIPAN4510板，孵育时由于毛细作用，试剂渗入膜中，此液体难以洗去，因此导致背景增加。为了避免此情况，建议在步骤12时将板底移去，将膜倒转，用蒸馏水流冲洗，同时用洗涤缓冲液按正常的步骤洗涤。在步骤14中，由于板底已移去，建议将MAIP4510板放于空的96孔板上，其后的步骤不变。
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