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- 运输方式：
冻存运输
- 相关疾病：
小细胞肺癌
- ATCC Number：
CRL-2062™
- 生长状态：
贴壁生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 年限：
54 years
- 库存：
大量
- 器官来源：
肺
- 细胞形态：
上皮样
| Designations: | DMS 53 | ||
| Depositors: | OS Pettengill, G Sorenson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: carcinoma; small cell lung cancer |
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| Cellular Products: | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF) |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications. | ||
| Isolation: | Isolation date: 1974 | ||
| Applications: | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy. The cells express HLA class I and class II antigens. |
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| Receptors: | bombesin, expressed epidermal growth factor (EGF), expressed transforming growth factor beta (TGF beta), expressed acetylcholine, expressed |
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| Tumorigenic: | Yes | ||
| Antigen Expression: | Leu 7; My23 | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 12,13 D5S818: 10,11 D7S820: 8,11 THO1: 8,9.3 TPOX: 12 vWA: 15,17 |
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| Age: | 54 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin derived products. |
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| Propagation: | ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: Keep cells heavy and subculture often. |
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| Subculturing: | Protocol:
Interval: Keep cells heavy and subculture often Subcultivation Ratio: 1:2 to 1:3 Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631 32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760 |
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文献和实验相关实验
DMS Chemical Footprinting of RNA
10X CE 9 mL H2O DMS Solution: 15 uL 100% ETOH 3 uL DMS Quench Solution: 1.4 mL 2-beta-mercaptoethanol 0.5 mL 3 M Sodium Acetate (Ambion
coco0663 P53 NM_000546.4 Transcript Variant: This variant (1) is a dominant transcript and encodes isoform a. 麻烦高手看下，这是野生型P53还是突变型P53？ Transcript Variant是否突变型的意思，那么NM_000546.4 所转录的是否就是突变型的P53？ 谢谢 侠骨仁医
whl111 P53蛋白具有肿瘤抑制作用，其机制可能是影响肿瘤细胞的凋亡。各位战友有没有想过如果将P53于体外直接作用于肿瘤细胞是不是会引起细胞的凋亡？而如果想办法让P53蛋白在体内能够接触到肿瘤细胞是不是可以起到抑制肿瘤细胞生长的作用呢？ pub_wang 不太可能，其通路在肿瘤发生中已被打破，肿瘤中整个平衡都变了。 5米 一颗种子当它还是种子的时候，你把种子煮一下也许再也不会发芽，当这颗
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DMS 53
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