- 询价
- 2026年01月09日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- 库存：
大量
- 年限：
68 years
- 相关疾病：
小细胞肺癌
- ATCC Number：
CRL-2066™
- 运输方式：
冻存运输
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | DMS 114 | ||
| Depositors: | OS Pettengill, G Sorenson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
||
| Source: | Organ: lung Disease: carcinoma; small cell lung cancer |
||
| Cellular Products: | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | epidermal growth factor (EGF); complement (CR3) | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | Leu 7 +; My23 +; CD11b + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 10,11 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 |
||
| Age: | 68 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
||
| Propagation: | ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
||
| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631 23142: Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验DMS Chemical Footprinting of RNA
10X CE 9 mL H2O DMS Solution: 15 uL 100% ETOH 3 uL DMS Quench Solution: 1.4 mL 2-beta-mercaptoethanol 0.5 mL 3 M Sodium Acetate (Ambion
Triton X-114 Phase Partitioning for Antigen Characterization
In 1981, Bordier (1 ) first demonstrated that Triton X-114 could be exploited as a means of separating hydrophilic and integral membrane proteins. Since then, Triton X-114 phase partitioning has been extensively used for identification
DMD(Duchenne Muscular Dystrophy)和BMD(Becker Muscular Dystrophy)是一种常见的X连锁隐性遗传病，主要发生于男性，其发病率为1/3500活产男婴，其发生的原因是Dystrophin(抗肌萎缩蛋)其固缺陷所致. 一、DMD/BMD的临床表现 在临床上DMD较BMD常见，发病早，症状重，正常于2-3岁即出现骨骼肌无力的表现，一般从骨盒带肌肉无力开始而出现一系列的特殊症状:走路困难呈鸭步
技术资料暂无技术资料 索取技术资料
