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- 详细信息
- 询价记录
- 技术资料
- 生长状态：
贴壁生长
- 库存：
大量
- 年限：
68 years
- 相关疾病：
小细胞肺癌
- ATCC Number：
CRL-2066™
- 运输方式：
冻存运输
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | DMS 114 | ||
| Depositors: | OS Pettengill, G Sorenson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: lung Disease: carcinoma; small cell lung cancer |
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| Cellular Products: | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | epidermal growth factor (EGF); complement (CR3) | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | Leu 7 +; My23 +; CD11b + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 10,11 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 |
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| Age: | 68 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
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| Propagation: | ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631 23142: Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655 |
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DMS 114
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