- 询价
- 2025年08月24日
14 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 年限：
85 yrs
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
上皮细胞癌
- ATCC Number：
CRL-2592™
- 组织来源：
epidermis
- 器官来源：
皮肤
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | A431NS | ||
| Depositors: | K Hirabayashi | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
||
| Source: | Organ: skin Tissue: epidermis Disease: epidermoid carcinoma |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 9,13 D16S539: 12,14 D5S818: 12,13 D7S820: 10 THO1: 9 TPOX: 11 vWA: 15,17 |
||
| Age: | 85 yrs | ||
| Gender: | female | ||
| Comments: | A431NS was derived from the A431 cell line (ATCC CRL-1555 ) in 1997 by repeated subculturing to select cells that detached from the substrate easily. The polyinosinic-polycytidylic acid/cationic liposome complex (LIC) inhibits cancer cell growth by the induction of apoptosis. [51526 ] LIC has strong cytotoxic effects on the A431NS cell line but neither polyinosinic-polycytidylic acid alone nor cationic liposome alone have antiproliferation effects. While cell sensitivity to drugs tend to be changeable, the A431NS cell line has a stable sensitivity to LIC. The cell line can be used to test the antiproliferation activity of LIC. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Protocol: Remove medium and rinse with PBS. Remove the PBS completely and add fresh 0.25% trypsin, 0.03% EDTA solution. Allow the flask to sit at room temperature (or 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperture |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-1555 |
||
| References: | 51526: Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
A431NS
询价
