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- 2026年01月08日
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- 文献和实验
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- 库存：
大量
- 细胞形态：
上皮样
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
皮肤
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 相关疾病：
黑色素瘤
- ATCC Number：
CRL-7898™
- 年限：
56 years
| Designations: | A101D | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: skin Disease: melanoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 11,12 D16S539: 12 D5S818: 12 D7S820: 10 THO1: 8,9.3 TPOX: 8,11 vWA: 16,18 |
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| Cytogenetic Analysis: | modal number = 63; range = 48 to 82 | ||
| Isoenzymes: | ADA, 1 ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1 |
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| Age: | 56 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A ratio of 1:4 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 |
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| References: | 23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 | ||
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文献和实验指DNA双链的局部，由具有互补性单链 DNA与之结合所产生的环状结构。当 DNA复制开始，在原来的双链中仅一方被新合成的短 DNA单链被置换的情况下可以见到（为 Displacement loop之简称）。可通过人工使 DNA单链结合来制成此结构。由 RNA单链所产生的类似结构称为 R环。
of each component.2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly for the large
of each component. 2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly
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