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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2020™
- 细胞类型：
其他细胞类型
- 运输方式：
冻存运输
- 库存：
大量
- 年限：
59 years
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- 器官来源：
大脑
| Designations: | DBTRG-05MG | ||
| Depositors: | CA Kruse | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: brain Disease: glioblastoma Cell Type: glial cell; |
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| Cellular Products: | vimentin; S-100 protein; neuron specific enolase | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | epidermal growth factor (EGF) | ||
| Antigen Expression: | Class I antigen expressed | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 9 D16S539: 10,12 D5S818: 12,13 D7S820: 11 THO1: 7,8 TPOX: 8 vWA: 15,16 |
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| Cytogenetic Analysis: | near tetraploid; range 87 to 91; most cells were missing copies of chromosome 10 and had extra copies of chromosome 7 | ||
| Age: | 59 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The DBTRG-O5MG (Denver Brain Tumor Research Group 05) cell line was established from tissue from a patient with glioblastoma multiforme who had been treated with local brain irradiation and multidrug chemotherapy. The cells are negative for platelet derived growth factor (PDGF), neuronal cell adhesion molecule (NCAM), glial fibrillary acid protein (GFAP) and class II antigen (HLA DR). No loss of heterozygosity in the p53 tumor suppressor gene was detected. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin, rinse and remove trypsin. Let the flask sit at room temperature (or incubate at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| References: | 24397: Kruse CA, et al. Characterization of a continuous human glioma cell line DBTRG-05MG: growth kinetics, karyotype, receptor expression, and tumor suppressor gene analyses. In Vitro Cell. Dev. Biol. 28A: 609-614, 1992. PubMed: 1331021 32274: Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765 32550: Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406 |
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文献和实验/V) 氯化镍/水贮存液, 30％ 过氧化氢, DPX, 光学显微镜(通常带有照相机以便于记录结果). 2. 操作步骤 (1) 用 9 mL 0.05 mol/L Tris 缓冲液 (pH7.6)溶解 6 mg DAB； (2) 加 l mL 0.3％(W/V)氯化镍/水贮存液(也可以用等量的氯化钴替代)； (3) 加0.1 mL 3％ 过氧化氢。过氧化氢一般以 30％ 的溶液提供，贮存在 4 ℃，此条件可以持续 1 个月； (4) 如果出现沉淀，用滤纸过滤； (5) 加此溶液至标本
How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
为 0.2 nM。当 Mg2+浓度过高时，因 Mg2+ 可与 dNTP 结合，适当加入多量的 dNTP 有助于 PCR 反应的进行。但过多的 dNTP 也会抑制 PCR 反应。当使用非校正 DNA 聚合酶时，降低 dNTP 的浓度（0.01-0.05 nM）及 Mg2+ 浓度有助于提高保真度。 【Mg2+】 Mg2+ 作为 DNA 聚合酶的辅助因子有助于 dNTP 的结合。在酶活性位点处的 Mg2+催化引物3’羟基和 dNTP 磷酸基团间磷酸二酯键的形成，另外 Mg2+ 可通过稳定磷酸基团上的负电
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