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![【ATCC来源】Neuro-2a [N2a; Neuro-2a]、Neuro-2a [N2a; Neuro-2a]、Neuro-2a [N2a; Neuro-2a]细胞、Neuro-2a [N2a; Neuro-2a]细胞、Neuro-2a [N2a; Neuro-2a]Neuro-2a [N2a; Neuro-2a][小鼠脑神经瘤细胞]](https://img1.dxycdn.com/2021/0721/054/1466071650690953943-14.jpg!wh200)
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
其他
- 年限：
54 years adult
- 相关疾病：
上皮细胞癌
- 运输方式：
冻存运输
- ATCC Number：
HTB-41™
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 库存：
大量
| Designations: | A-253 | ||
| Depositors: | G Todaro | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: submaxillary salivary gland Disease: epidermoid carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12,13 D13S317: 9,12 D16S539: 12 D5S818: 11 D7S820: 10,12 THO1: 6,8 TPOX: 8,12 vWA: 16 |
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| Cytogenetic Analysis: | near triploid with at least 6 markers | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1 PGM3, 1 |
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| Age: | 54 years adult | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The A253 line is one of thirteen to be isolated and partially characterized through trial studies on a series of 200 human tumors. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.53 mM EDTA solution, remove the trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Interval: every 6 to 8 days Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| References: | 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 |
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文献和实验化反应。例如氢分子离解成氢原子时，如果用波长 253.7nm 的汞蒸气灯去照射氢气，并不发生反应。若在氢气中加入一点汞蒸气，然后把此混合气体暴露在 253.7nm 波长的光中，氢分子就立刻进行离解，这里的汞蒸气就是光敏剂。汞原子吸收光子变为激发态原子，激发态汞原子遇到氢分子时，前者就将吸收的能量传给后者，使后者离解。上述过程可表示为 如果没有光敏剂的存在，要在光照下直接产生离解反应 H 2 hv → 2H ，则要求光的波长λ≤ 84.9nm ，即要求光子的能量
, The Zebrafish Book , or Kimmel et al., Develop. Dynam. 203:253-310.
Cell Lineage Analysis: X-Inactivation Mosaics
phenomenon, known as X inactivation or lyonization, provides an ideal nonsurgical method of producing mosaicism among otherwise identical cells of the embryo. We have created a line of mice (H253) by pronuclear injection of a DNA fragment containing the lacZ
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