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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫类型：
IgM
- 细胞形态：
淋巴样
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 细胞类型：
B淋巴细胞
- 器官来源：
外周血
- 年限：
16 years
- 生长状态：
悬浮生长
- 相关疾病：
淋巴瘤
- ATCC Number：
CCL-213™
- 运输方式：
冻存运输
| Designations: | Daudi | ||
| Depositors: | G Klein | ||
| Isotype: | IgM | ||
| Biosafety Level: | 2 [Cells Contain HERPESVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Disease: Burkitt's lymphoma Cell Type: B lymphoblast; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: May, 1967 | ||
| Applications: | transfection host | ||
| Receptors: | complement, expressed Fc, expressed |
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| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 12 D13S317: 11,12 D16S539: 10,12 D5S818: 8,13 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 15,17 |
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| Cytogenetic Analysis: | Male human karyotype with stemline number of 46. The karyotype is diploid in 66% of the cells and is stable within the stemline. | ||
| Isoenzymes: | G6PD, B | ||
| Age: | 16 years | ||
| Gender: | male | ||
| Ethnicity: | Black | ||
| Comments: | The Daudi line was derived from a 16-year-old Black male with Burkitt's lymphoma by E. Klein and G. Klein in May, 1967. The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin (sIg+). The line carries Epstein-Barr virus. The Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 10(5) viable cells/ml. Interval: Maintain cell density between 3 X 10(5) and 2 to 3 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 23017: Klein E, et al. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 28: 1300-1310, 1968. PubMed: 4174339 26046: Huber C, et al. Surface receptors on human haematopoietic cell lines. Clin. Exp. Immunol. 25: 367-378, 1976. PubMed: 963908 26047: Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896 28315: Gao Y, et al. Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurkitts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. PubMed: 8985326 32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 32830: Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 33091: Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694 33115: Montoya JG, et al. Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect. Immun. 64: 176-181, 1996. PubMed: 8557337 |
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