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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
骨肉瘤
- ATCC Number：
CRL-1427™
- 器官来源：
骨
- 库存：
大量
- 生长状态：
贴壁生长
- 年限：
14 years
- 细胞形态：
成纤维样
| Designations: | MG-63 | ||
| Depositors: | A Billiau | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: bone Disease: osteosarcoma |
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| Cellular Products: | interferon | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Receptors: | transforming growth factor beta (TGF-beta) RI, expressed transforming growth factor beta (TGF-beta) RII, expressed |
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| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 11 D5S818: 11,12 D7S820: 10 THO1: 9.3 TPOX: 8,11 vWA: 16,19 |
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| Cytogenetic Analysis: | This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells. | ||
| Age: | 14 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 | ||
| References: | 1058: Billiau A, et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. PubMed: 883813 23014: Takeuchi Y, et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639 32445: Yee A, et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. PubMed: 8550604 |
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文献和实验摘 要一、人成骨肉瘤MG-63细胞株和成人成骨细胞的培养与分化特性的观察目的：观察人成骨肉瘤MG-63细胞株和正常成人成骨细胞分化的特性。方法：在细胞培养的不同时间，用α-磷酸奈酚法测定碱性磷酸酶（ALP）活性；放射免疫法测定骨钙素（BGP）含量；半定量逆转录聚合酶链反应（RT-PCR）测I型胶原、MMP-1、TIMP-1基因mRNA表达；Van GieSon氏苦味酸酸性复红染色法染色细胞I型胶原。结果：人成骨肉瘤MG-63细胞株接种培养第7天后I型胶原基因表达较高。MMP-1表达量随时间
当师弟还在为有没有钱途，而纠结明年考不考博时，小编也不好替他拿主意。但看到一份文件后，小编立刻告诉他：你要读博！要去 G 省读！毕业后留在 G 省工作！ 究竟是什么文件，让小编瞬间认为读博很有钱途？ G 省又是哪个厚待人才的大省？广东人才计划原来是广东省出台的一份《关于加快新时代博士和博士后人才创新发展的若干意见》的通知，这份文件究竟有哪些吸引人的地方呢？ 1. 每年补贴 200 名博士博后，每人 50 万，对，是生活补贴，可不是啥课题经费，有木有心动？小编是心动了。 2. 再看这一条
CD63 分子 CD63 常用单克隆抗体或代号： RUU- SP2.28,CLB 主要表达细胞： Pt,M,Mac,(G,T,B) [Pt] 分子质量(kDa)和结构： gp53,血小板颗粒蛋白(TM4- SF) 功 能： 血小板活化,中性粒细胞- 活化内皮细胞粘附 CD63 Aka lysosomal-membrane-associated glycoprotein 3, LAMP
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