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- 运输方式：
冻存运输
- 库存：
大量
- 器官来源：
子宫
- 细胞形态：
成纤维样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1976™
- 年限：
56 years
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
| Designations: | MES-SA | ||
| Depositors: | BI Sikic | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: uterus Disease: uterine sarcoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1980 | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 11,13 D16S539: 11,12 D5S818: 13 D7S820: 7,11 THO1: 6 TPOX: 8,11 vWA: 18 |
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| Cytogenetic Analysis: | This is a hypo-diploid human cell line with the modal chromosome number of 45 in 48% of cells examined. The rate of polyploidy was 2.7%. There were five or six marker chromosomes present in each cell. Whereas t(5q6p), 10q+, and 14q+ markers were common in all cells examined, the chromosome combination of either double copy N21 lacking M5 (66.7%) or t(21qter--->C--->?)(M5)/single copy N21/minute metacentric (33.3%) distinguishes the two coexisting clones. Both X chromosomes were normal. | ||
| Age: | 56 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The MES-SA cell line was established from a surgical tumor specimen obtained at the time of hysterectomy. The tumor was described as a poorly differentiated uterine sarcoma. Initially, the cells were grown in soft agar, and later they were transferred to muliwell plates. The nonepithelial origin of the cells was supported by ultrastructural studies and the absence of staining for mucin. The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine, dacarbazine, cisplatin, melphalan, vincristine, methotrexate and etoposide. The multiple drug resistant cell line MES-SA/Dx5 (ATCC CRL-1977 ) was established from MES-SA cells which were grown in the presence of doxorubicin. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Growth Conditions: Newborn calf serum may be substituted for fetal bovine serum. |
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| Subculturing: | Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 22 to 24 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007 recommended serum:ATCC 30-2020 derivative:ATCC CRL-1977 derivative:ATCC CRL-2274 |
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| References: | 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344 | ||
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